Cellular Rspiration

LABORATORY REPORT FOR BIO411 test 5 Cellular Respiration Experiment 6 Photosynthesis Title Cellular Respiration Objective To observe and pin down cellular ventilating system in yeast/onion cells. * Measure respiration assess exploitation different substrates. * Measure respiration rate at different temperature. Introduction In this laboratory experimentation, we are given 3 task. The jump one is respiration in yeast. Second is respiratory index number and the third one is observing mitochondria in yeast/onion cell. The first experiment is about investigation of sugars yeast. We volition determine which sugars yeast give notice be use for cell respiration.When the yeast undergoes anaerobic/aerobic respiration, they will give out carbon dioxide. After that it will reacts with pee to forms a weak carbonic acid resolve. We will use BTB to monitor this reaction. yet explanation will be discussed at the discussion. The second experiment is respiratory indicator. Same concep t with the first experiment. We will measure the respiration rate development different substrates based on the table provided. The third experiment is observing mitochondria in yeast/onion cells. We will be using the staining technique in order to obtain the result of the experiment. * line of work 1 Respiration in yeast Materials Beakers * Pipettes * Cuvettes * 20% Glucose * 20% lactose * 20% saccharose * 20% Maltose * Distilled water * Brom Thymol ghastly (BTB) * Spectrophotometer * Measuring cylinders. Procedures 1. The spectrometer is set to 565nm. Distilled water is used to set the denotation to the pure level. 2. 8mL of 20% glucose is placed in the beaker using a measurement cylinder. 3. 1ml of Brom Thymol Blue (BTB) is added into a respective tubes using a pipette. 4. 4ml from the categorization of Glucose and BTB interpreted and placed in test tube then added with 0. 1ml of yeast extract. 5. The mixture then transferred into cuvette and placed into spectrophotometer. 6.The absorbance measured for 5 minutes. The reading taken within 30 seconds. 7. Steps 1-6 is repeated using 20% Lactose, 20% Sucrose, and 20% Maltose. 8. Reading obtained is recorded and compared with the others. Results m (s) 20% Glucose 20% Sucrose 20% Maltose 20% Lactose 30 1. 053 1. 162 1. 323 1. 130 60 1. 051 1. 161 1. 310 1. 123 90 1. 049 1. 159 1. 304 1. 119 120 1. 048 1. 159 1. 297 1. 116 150 1. 045 1. 158 1. 295 1. 114 180 1. 044 1. 155 1. 292 1. 111 210 1. 041 1. 155 1. 289 1. 109 240 1. 040 1. 154 1. 287 1. 107 270 1. 037 1. 154 1. 287 1. cv 300 1. 035 1. 154 1. 284 1. 103 Task 2 Respiratory Indicator Materials * Test tubes * Yeast extract * 20% Glucose * Water bath * Parafilm * Distilled water * Tap water * Methylene no-account Procedures 1. 4 test tubes is obtained. 2. Each tube fill up as table shown below, tubing 1 (room temperature) Tube 2 (100? c) Tube 3 Tube 4 5ml yeast+1ml glucose+2ml methylene down in the mouth+2ml distilled water 5ml yeast+1ml glucose+2m l methylene blue+2ml distilled water 5ml water+1ml glucose+2ml methylene blue+2ml distilled water 5ml yeast+1ml water+1ml methylene blue +2ml distilled water 3. For tube 2, yeast is added and glucose is immersed the tube in water bath(100%) for 5 minutes.The, methylene blue is added. 4. Parafilm is used to cover all the tubes. 5. Initial time and color is recorded. 6. Time taken for the decolorisation to occur is recorded. Result Tube 1 Tube 2 Tube 3 Tube 4 Observation juicy blue(no change) Dark blue(no change) Dark blue(no change) Dark blue(no change) * Task 3 Observing Mitochondrian of Onion Cells Materials * glass over slides * Sucrose solution * Methylene blue * Toothpick * Onion cell * expand slip * Microscope Procedures 1. Clean slide is obtained and a drop of sucrose solution is placed on the center.Two drops of methylene blue added and mixed well by using toothpick. 2. Yeast is placed on the mixture of sucrose and methylene blue and cobered by the cover slip. 3. The slid e immediately viewed under microscope. Results 35 minutes is taken for yeast cells to decolorized, where blue stain turns clear. Disccusion In our experiment, in order to achieved our objective which are to observe and determine cellular repiration in yeast/onion cells, we have to measured respiration rate using different substrates and we also have to measure respiration rates at different temperatures in Task 1, Task 2, Task 3.In Task 1 Respiration in yeast, the result we obtained shows that the 20% Glucose give the lowest measure out of absorbance compared to the others and 20% Maltose gave the highest value of absorbance. The order of absorbance is 20% Glucose